Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code APP-101
Description Peste-des-petits-ruminants (PPR) is an acute viral disease of small ruminants characterised by fever, oculonasal discharges, stomatitis, diarrhoea and pneumonia with foul offensive breath. It is caused by a Morbillivirus that belongs to the family Paramyxoviridae. PPR affects mainly sheep and goats and occasionally wild small ruminants. It has been reported on a few occasions in camels, cattle and buffaloes, although their potential role in the circulation of PPR virus (PPRV) is not established.

PPR occurs in Africa except Southern Africa, in the Arabian Peninsula, throughout most of the Near East and Middle East, and in Central and South-East Asia. The clinical disease resembles rinderpest in cattle. It is usually acute and characterised by pyrexia, serous ocular and nasal discharges, erosive lesions on different mucous membranes particularly in the mouth, diarrhoea and pneumonia.

Method Real -Time RT-PCR
Sample Type
Swab / Secretion (Respiratory), Culture, EDTA Blood, Tissue.
Transport Condition Samples should be transported at 4°C and delivered within 24 hours. It is required to add RNA preservative media to swabs / secretion and tissue only.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APC-005
Description Beak and feather disease virus or Circovirus is a small, non-enveloped circular single-stranded DNA virus of the Circoviridae family. The virus causes beak and feather disease (PBFD) in avian species specifically psittacine birds such as Cockatoos, Macaws, African grey parrots and Ringnecked parakeets. Beak and feather disease virus typically targets actively growing cells like beak, feather, and the cells of the immune system resulting in secondary bacterial, fungal or parasitic infections. The viral particles remain viable in the environment for months.

Transmission of the virus from one individual to another is through direct contact, inhalation of feather dust or ingestion of infected faecal material. Clinical signs of the disease include irreversible loss of feathers, shedding of developing feathers, development of abnormal beak or feathers, and lesions on the beak and nails.

There is no known treatment and the only way to control the disease is through hygiene, strict isolation or culling of all diseased birds. Early diagnosis is necessary to control disease progression in infected birds and avoid its spread amongst uninfected ones.

Method Real -Time PCR
Sample Type
Accredited : EDTA Blood, Tissue, Culture.
Alternatives : Feather.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code APP-101
Description Peste-des-petits-ruminants (PPR) is an acute viral disease of small ruminants characterised by fever, oculonasal discharges, stomatitis, diarrhoea and pneumonia with foul offensive breath. It is caused by a Morbillivirus that belongs to the family Paramyxoviridae. PPR affects mainly sheep and goats and occasionally wild small ruminants. It has been reported on a few occasions in camels, cattle and buffaloes, although their potential role in the circulation of PPR virus (PPRV) is not established.

PPR occurs in Africa except Southern Africa, in the Arabian Peninsula, throughout most of the Near East and Middle East, and in Central and South-East Asia. The clinical disease resembles rinderpest in cattle. It is usually acute and characterised by pyrexia, serous ocular and nasal discharges, erosive lesions on different mucous membranes particularly in the mouth, diarrhoea and pneumonia.

Method Real -Time RT-PCR
Sample Type
Swab / Secretion (Respiratory), Culture, EDTA Blood, Tissue.
Transport Condition Samples should be transported at 4°C and delivered within 24 hours. It is required to add RNA preservative media to swabs / secretion and tissue only.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APC-005
Description Beak and feather disease virus or Circovirus is a small, non-enveloped circular single-stranded DNA virus of the Circoviridae family. The virus causes beak and feather disease (PBFD) in avian species specifically psittacine birds such as Cockatoos, Macaws, African grey parrots and Ringnecked parakeets. Beak and feather disease virus typically targets actively growing cells like beak, feather, and the cells of the immune system resulting in secondary bacterial, fungal or parasitic infections. The viral particles remain viable in the environment for months.

Transmission of the virus from one individual to another is through direct contact, inhalation of feather dust or ingestion of infected faecal material. Clinical signs of the disease include irreversible loss of feathers, shedding of developing feathers, development of abnormal beak or feathers, and lesions on the beak and nails.

There is no known treatment and the only way to control the disease is through hygiene, strict isolation or culling of all diseased birds. Early diagnosis is necessary to control disease progression in infected birds and avoid its spread amongst uninfected ones.

Method Real -Time PCR
Sample Type
Accredited : EDTA Blood, Tissue, Culture.
Alternatives : Feather.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links